CST, 8787S, ER-Tracker Green (BODIPY FL Glibenclamide)

Product Usage Information
ER-Tracker Green (BODIPY FL Glibenclamide) is supplied as a single 100 μg lyophilized vial. To make a 1 mM stock solution, reconstitute the solid in 128 μl of high quality DMSO. Dilute 1:500 directly into normal growth media for a working concentration of 2 μM and analyze immediately. Cells must be imaged live; DO NOT FIX! To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging.
Storage
Store lyophilized solid or solution at -20°C dessicated and protected from light. Once reconstituted in DMSO, the solution should be aliquoted to avoid freeze-thaw cycles. If stored properly, this reagent is stable for 6 months.
Background
ER-Tracker Green (BODIPY FL Glibenclamide) is a cell-permeant, live cell stain that is highly selective for the endoplasmic reticulum (ER). This stain consists of the green-fluorescent BODIPY FL dye and glibenclamide. Glibenclamide (glyburide) binds to sulphonylurea receptors, the regulatory subunit of ATP-sensitive K channels prominently expressed in the ER. The pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.
Alternate Names
Endoplasmic; ER; ER-Tracker; ERTracker; live cell; live-cell; Reticulum