CST, 8961S, ProLong Gold Antifade Reagent with DAPI

Product Usage Information
Ensure product is brought to RT prior to use. Following immunostaining, cover samples with ProLong Gold Antifade Reagent with DAPI # 8961 and coverslip. To prevent fading, allow slides to cure in the dark, at room temperature for 24 hours, then seal the coverslips with clear lacquer, such as nail polish. Proceed with imaging.
Storage
Store at -20°C. ProLong ® Gold Antifade Reagent is stable for 12 months when stored protected from light, at or below -20°C.
Background
ProLong Gold Antifade Reagent with DAPI offers enhanced resistance to photobleaching, is premixed and ready to use, and causes little or no quenching of the fluorescent signal. The reagent cures within 24 hours and samples can be saved for months after mounting. This reagent is compatible with most dyes and is appropriate for use in multi-color labeling assays. The inclusion of DAPI within this mounting reagent eliminates the need for a separate nuclear counterstaining step. 4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content (3).
Alternate Names
DAPI; Mounting; Prolong Gold