CST, 89726S, Lambda Protein Phosphatase Kit

Product Usage Information
1. Add protein sample and H 2 O (if necessary) to make a total volume of 40 μL. 2. Add 5 μL of 10X Buffer for Protein MetalloPhosphatases (PMP) and 5 μL of 10 mM MnCl2 for PMP to make a total reaction volume of 50 μL. 3. Add 1 μL of Lambda Protein Phosphatase. 4. Incubate at 30°C for 30 min. Note: The optimal incubation times and enzyme concentrations must be determined empirically for each individual substrate.
Storage
All components in this kit are stable for 24 months when stored at the recommended temperature. Aliquot to avoid repeated freeze/thaw cycles.
Specificity / Sensitivity
The Lambda Protein Phosphatase Kit is active toward phosphorylated serine, threonine, and tyrosine residues.
Source / Purification
The Lambda Protein Phosphatase component is produced in a strain of that carries the bacteriophage lambda ORF221 open reading frame. The product is isolated using the T7 expression system.