Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Acetyl-beta-Catenin (Lys49) (D7C2) Rabbit Monoclonal Antibody recognizes endogenous levels of β-catenin protein only when acetylated at Lys49.
Species Reactivity: Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys49 of human β-catenin protein.
Background
β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). Lys49 lies in a region that contains several Ser/Thr residues whose phosphorylation status regulates the stability of β-catenin. Research studies have shown that Lys49 is one of few residues frequently mutated in thyroid anaplastic carcinoma (9). CREB-binding protein (CBP) binds and acetylates β-catenin at Lys49 (10,11).
Alternate Names
armadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta 1; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; EVR7; FLJ25606; FLJ37923; MRD19; NEDSDV
