CST, 9074S, MitoTracker ® Green FM

Product Usage Information
Each vial of MitoTracker ® Green FM contains 50 µg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 74.4 µl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results are obtained by diluting the stock solution directly into normal growth media at a concentration between 100-400 nM and incubating between 15-30 min at 37ºC. After incubation, the cells must be imaged live; DO NOT FIX! To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging.
Storage
Store lyophilized solid at -20°C dessicated and protected from light. In lyophilized form, this reagent is stable for 12 months. Once reconstituted in DMSO, the solution should be stored at -20°C, protected from light, and must be used within 2 weeks. Avoid freeze-thaw cycles.
Background
MitoTracker dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.
Alternate Names
cell; FM; greeen; immunofluorescence; live; mito; Mito-Tracker; Mitochondria; MitoTracker; tracker