Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
alpha-Tubulin (11H10) Rabbit Monoclonal Antibody (HRP Conjugate) detects endogenous levels of total α-tubulin protein and does not cross-react with recombinant β-tubulin protein.
Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster, Zebrafish, Bovine, Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human α-tubulin protein.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Alternate Names
alpha tubulin; Alpha-tubulin ubiquitous; Detyrosinated tubulin alpha-1B chain; K-ALPHA-1; TBA1B; TUBA1B; tubulin alpha 1b; Tubulin alpha-1B chain; Tubulin alpha-ubiquitous chain; Tubulin K-alpha-1; tubulin, alpha 1b; tubulin, alpha, ubiquitous
