Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
ADK (F5Q5P) Rabbit Monoclonal Antibody recognizes endogenous levels of total ADK protein.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ADK protein.
Background
Adenosine kinase (ADK) is an evolutionary conserved ribokinase that functions as a key regulator of adenosine metabolism by catalyzing the phosphorylation of adenosine to produce 5-adenosine monophosphate (AMP) (reviewed in 1). Adenosine is not only a critical component of RNA, but also functions in maintaining energy homeostasis as a precursor of ATP production, second messenger signaling through the production of cAMP, maintaining S-adenosylmethionine-dependent transmethylation and adenine-containing co-enzymes NAD and FAD, and can directly signal through its cognate receptors. ADK has two isoforms with distinct expression and subcellular localization: a nuclear long form (ADK-L) and a cytoplasmic short form (ADK-S) (2). Genetic knockout of ADK in mice results in perinatal lethality, microvesicular hepatic steatosis, and stunted growth (3). ADK dysfunction is involved in several pathologies, including diabetes, epilepsy, and cancer (4).
Alternate Names
Adenosine 5'-phosphotransferase; Adenosine kinase; ADK; AK; testicular tissue protein Li 14
