Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
AHCYL1/IRBIT (D3A5G) Rabbit Monoclonal Antibody recognizes endogenous levels of total AHCYL1/IRBIT protein. This antibody does not cross-react with AHCYL2/IRBIT2 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu50 of human AHCYL1/IRBIT protein.
Background
S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1) is a member of S-adenosylhomocysteine hydrolase family, which participates in the metabolism of S-adenosyl-L-homocysteine (1). Two homologs of S-adenosylhomocysteine hydrolase-like proteins, dAhcyL1 and dAhcyL2, were identified as novel components of methionine metabolism (2) dAhcyL1 and dAhcyL2 function as dominant-negative regulators of S-adenosylhomocysteine hydrolase (2). Global down-regulation of both dAhcyL1 and dAhcyL2 extended life span (2). In addition, brain-specific down regulation of dAhcyL1 extended life span (2). AHCYL1 is also known as inositol 1,4,5-trisphosphate receptor (IP R) binding protein released with IP (IRBIT) (1, 3). This protein binds to the endoplasmic reticulum calcium release channel IP R and represses its acitivity (1, 3). As a multifunctional regulator, AHCYL1/IRBIT can also form a complex with and suppress the activity of ribonucleotide reductase, thereby influencing the balance of deoxynucleotide triphosphates essential for DNA replication and genomic integrity (4).
Alternate Names
adenosylhomocysteinase 2; adenosylhomocysteinase like 1; adenosylhomocysteinase-like 1; AdoHcyase 2; AHCYL1; DC-expressed AHCY-like molecule; DCAL; dendritic cell expressed AHCY-like protein; epididymis secretory sperm binding protein; inositol 1,4,5-trisphosphate receptor-binding protein; IP(3)Rs binding protein released with IP(3); IRBIT; PPP1R78; PRO0233; protein phosphatase 1, regulatory subunit 78; Putative adenosylhomocysteinase 2; S-adenosyl homocysteine hydrolase homolog; S-adenosyl-L-homocysteine hydrolase 2; S-adenosylhomocysteine hydrolase-like 1; S-adenosylhomocysteine hydrolase-like protein 1; SAHH2; XPVKONA
