Description
Product Usage Information
Cytochalasin D is supplied as a lyophilized powder. For a 5 mM stock, reconstitute 1 mg of powder in 0.39 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Storage
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles .
Background
The fungal-derived toxin Cytochalasin D is a potent inhibitor of actin polymerization, limiting elongation at both the barbed and pointed ends of the actin filament (1). While several related Cytochalasin toxins bind actin and alter polymerization, Cytochalasin D is the most specific inhibitor of actin microfilaments (2). The cytoskeleton takes part in signal transduction, protein transport, signal compartmentalization, and cell mobility. Depolymerization of the actin cytoskeleton by Cytochalasin D in mesenchymal stem cells results in localization of G-actin to the nucleus, which leads to mesenchymal stem cell differentiation and bone formation (3). Treatment of a pair of cancer cell lines with Cytochalasin D results in the inhibition of actin polymerization and decreased cell motility (4). Treatment of murine CT26 colorectal carcinoma cells with Cytochalasin D inhibited tumor growth through reduced cell proliferation, induction of cell apoptosis, and suppression of tumor angiogenesis (5).
Alternate Names
(7S,13E,16S,18R,19E,21R)-21-(Acetyloxy)-7,18-dihydroxy-16,18-dimethyl-10- phenyl[11]cytochalasa-6(12),13,19-triene-1,17-dione
