Description
Product Usage Information
Immunofluorescence (Frozen): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunofluorescence (Frozen)
Specificity / Sensitivity
ALDH1L1 (E7I2Q) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of ALDH1L1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
This recombinant chimeric antibody is engineered from ALDH1L1 (E7I2Q) Rabbit mAb #85828 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, ALDH1L1 (E7I2Q) Rabbit mAb #85828, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val431 of human ALDH1L1 protein.
Background
Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), also known as 10-formyltetrahydrofolate dehydrogenase (FDH), is a member of the aldehyde dehydrogenase super family. It is a key enzyme in folate metabolism, playing an important role in regulation of cellular metabolism including proliferation (1,2). Downregulation of ALDH1L1 has been seen in tumors, leading to decreased suppression of cancer cell proliferation (2,3). ALDH1L1 has also been shown to be a useful astrocyte marker throughout the gray and white matter of the brain, labeling both the cell body and processes of astrocytes. ALDH1L1 does not label oligodendrocytes or neurons (4). It has been shown that expression of ALDH1L1 in astrocytes is stably expressed through normal tissue and during astrogliosis (5).
Alternate Names
10-formyltetrahydrofolate dehydrogenase; 10-fTHF; 10-FTHFDH; AL1L1; aldehyde dehydrogenase 1 family member L1; aldehyde dehydrogenase 1 family, member L1; aldehyde dehydrogenase 1L1; Aldehyde dehydrogenase family 1 member L1; ALDH1L1; Cytosolic 10-formyltetrahydrofolate dehydrogenase; DKFZp781N0997; FDH; formyltetrahydrofolate dehydrogenase; FTHFD; MER57B-ALDH1L1
