Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
AP3B1 (E7U2P) Rabbit Monoclonal Antibody recognizes endogenous levels of total AP3B1 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human AP3B1 protein.
Background
The adaptor protein complex 3 (AP-3) is a ubiquitously expressed, heterotetramer that is comprised of two large adaptins (AP3D1 and either AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2), and a small adaptin (AP3S1 or AP3S2). The AP-3 complex is involved in the sorting of protein cargo within the endosomal compartment that are destined for lysosomes (1,2). Defects in the AP-3 complex have been associated with Hermansky-Pudlak syndrome (HPS), a genetic disorder characterized by abnormal skin pigmentation, blood clotting abnormalities, and pulmonary fibrosis. Mutations in the gene encoding ubiquitously expressed AP3B1/β3A, destabilize the protein and the entire AP-3 complex, which results in defective trafficking of lysosomal membrane proteins (3,4). Research studies have shown that certain viral proteins have evolved to co-opt the AP-3 complex by binding to AP3B1 in order to facilitate viral particle assembly (5).
Alternate Names
Adapter-related protein complex 3 subunit beta-1; Adaptor protein complex AP-3 subunit beta-1; adaptor related protein complex 3 beta 1 subunit; adaptor related protein complex 3 subunit beta 1; Adaptor-related protein complex 3 subunit beta-1; adaptor-related protein complex 3, beta 1 subunit; ADTB3; ADTB3A; AP-3 complex beta-3A subunit; AP-3 complex subunit beta-1; AP3B1; Beta-3A-adaptin; beta3A-adaptin; Clathrin assembly protein complex 3 beta-1 large chain; HPS; HPS2; PE
