CST, 95978S, BODIPY 581/591 C11 (Lipid Peroxidation Sensor)

Product Usage Information
Supplied Reagent: 1. BODIPY 581/591 C11 (Lipid Peroxidation Sensor): Create a 10 mM stock solution by dissolving 1 mg of BODIPY 581/591 C11 (Lipid Peroxidation Sensor) into 198.26 μL of high-quality anhydrous DMSO. Additional Reagents (Not Supplied): 1. Hanks' Balanced Salt Solution (HBSS) 2. DMSO (Dimethyl Sulfoxide), Sterile # 12611 Protocol: 1. Incubate cells with 1 - 2 µM of BODIPY 581/591 C11 (Lipid Peroxidation Sensor) and 1 μM of Hoechst 33342 # 4082 (optional) in cell culture media for 30 min. 2. Wash two times with HBSS. 3. Add desired treatments in HBSS and incubate as needed. 4. Examine cells by fluorescence microscopy. Refer to the Fluorescent Properties section for excitation and emission spectra. 5. For analysis by flow cytometry, dissociate adherent cells or collect suspension cells. Viability dye can be used if desired. Analyze on a flow cytometer at desired cell density.
Storage
Store lyophilized at -20°C. In lyophilized form, the product is stable for 12 months.