CST, 97202S, Human IL-6R-alpha Recombinant Protein

Storage
Human IL-6Rα Recombinant Protein is supplied as lyophilized material that is very stable at -20ºC. It is recomended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Source / Purification
Recombinant hIL-6Rα was expressed in 293 cells and is supplied in a lyophilized form.
Background
The interleukin-6 (IL-6) receptor is a heterodimeric complex that consists of a ligand-binding IL-6 receptor α (IL-6Rα) subunit and a signaling component, gp130 (1). Binding of IL-6 to IL-6Rα results in dimerization of receptor with gp130 and subsequent STAT3 activation (1). IL-6Rα is cleaved from the cell surface by ADAM17 (1,2). In humans, soluble IL-6Rα is also generated via alternatively spliced mRNA (1,3). Soluble IL-6Rα binds to IL-6 and can stimulate signaling via membrane-bound gp130 in a process known as "trans-signaling" (1). Through trans-signaling, IL-6 stimulates cells that do not express membrane-bound IL-6Rα (1).