CST, 9802S, Kinase Buffer (10X)

Product Usage Information
1. Thaw 10X buffer at 24-30°C, mixing end-over-end. 2. Dilute the 10X buffer to the desired concentration using ddH2O.
Storage
1X Kinase Buffer: 25 mM Tris-HCl (pH 7.5), 5 mM beta-glycerophosphate, 2 mM dithiothreitol (DTT), 0.1 mM Na3VO4, 10 mM MgCl2. Note: Kinase Buffer must be supplemented with ATP (#9804). For long term storage, keep 10X buffer at -20°C If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. Aliquoting of 10X buffer is recommended if many small experiments are to be performed. 1X solution can also be aliquotted into single use aliquots and stored at -20°C.