Cytiva, 11001303, HiTrap Capto Q, 5 × 5 mL

Name: HiTrap Capto Q, 5 × 5 mL
Brand: Cytiva
SKU: 11001303

Catalog Number: 11001303

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Category

Protein Purification Columns

Pack Size5 mL

TagsPrepacked columns Chromatography products Ion Exchange

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Protein capture and intermediate purification with Capto Q resin Capto Q resin couples a quaternary ammonium (Q) strong anion exchanger to a chemically modified, high-flow agarose matrix. The rigid agarose matrix provides greater particle rigidity without compromising pore size, which allows for high bed volume and purification of viscous samples at high flow rates. High dynamic binding capacity is achieved with a dextran surface extender that coats the agarose matrix. The resin’s high binding-capacity, high flow rate, and low back pressure reduce process cycle times and improve productivity at process scale. Successful use in published research Research applications for this strong anion exchange resin include: The effects of a plant-produced vaccine candidate on Plasmodium falciparum transmission Analysis of outer membrane targeting of secretin PuID protein, and reliance on a dedicated chaperone Molecular characterization of Plasmodium falciparum uracil DNA glycosylase Other Capto Q resin formats The resin in this prepacked column is also available as bulk resin and in other prepacked column formats. Therefore, it is possible to use the same resin for development work, pilot studies, and routine production. Other Cytiva ion exchange products Among Cytiva's solutions are a variety of ion exchange chromatography products, including, Capto, Sepharose, and SOURCE.

FAQs

Question: What is the pack configuration?

Answer:Five separate HiTrap Capto Q columns, each with a 5 mL resin bed, are supplied.

Question: What mechanism drives binding on these columns?

Answer:Strong anion exchange via quaternary ammonium ligands retains appropriately negatively charged analytes under controlled pH and ionic-strength conditions.

Question: What makes this format useful during development?

Answer:The 5 mL prepacked beds allow repeatable capture or intermediate-purification experiments without packing chromatography columns in-house.

Application Note

HiTrap Capto Q chromatography columns are designed for protein purification by strong anion exchange. This pack supplies five prepacked 5 mL columns containing Capto Q resin with quaternary ammonium binding groups. The format allows systematic evaluation of loading capacity, buffer conductivity and salt-elution conditions in larger small-scale preparations. Negatively charged proteins can bind when the mobile-phase conditions favor electrostatic interaction with the ligand, while other components may pass through or elute differently. Analyze collected fractions for recovery, purity and aggregate content as relevant to the purification objective. The package has a total of 25 mL of prepacked resin distributed among five separate columns, not a single 25 mL bed.

Ordering

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