Cytiva, 17044401, Lentil Lectin Sepharose 4B, 25 mL
Catalog Number: 17044401
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Pack Size25 mL
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| Application | For purification, isolation or removal of molecules containing branched mannoses with fucose linked α(1,6) to the N-acetylglucosamine, (αMan>αGlc>GlcNAc) and sterically related residues like glycoproteins, membrane proteins, glycolipids, lipoproteins, pol |
|---|---|
| Chemical stability | Stable to commonly used aqueous buffers. Chelating agents such as EDTA, 8 M urea or solutions having a pH below 3 should be avoided as these conditions result in removal of manganese from the lectin and loss of activity. |
| Flow velocity | < 75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height |
| Ligand | Lentil lectin |
| Ligand Coupling Method | Cyanogen bromide activation |
| Ligand density | 2 mg Lentil lectin/ml medium |
| Matrix | Agarose, 4% |
| Particle Size | 45 µm-165 µm |
| Particle size, d 50V | ~90 µm |
| Pressure/Flow Specification | Base matrix 70-140 cm/h, pressure drop cm H 2 O/bed height=15, bed height 10 cm, 5 cm i.d. |
| Storage | 2 to 8°C, 20% Ethanol, 0.9% NaCl, 1 mM Ca 2+ and 1 mM Mn 2+ |
| pH stability, CIP | 3–10 |
| pH stability, operational | 3–10 |
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