Cytiva, 17050001, DEAE Sephacel, 500 mL
Catalog Number: 17050001
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Category
Pack Size500 mL
| Chromatography technique | Ion Exchange Chromatography |
|---|---|
| Autoclavable | In 0.1 M NaCl at 121°C for 30 min |
| Avoid Using | Oxidizing materials |
| Binding Capacity/ml Chromatography Medium | 10 mg Thyroglobulin (MW 669 000)/mL drained resin, 160 mg HSA (MW 68 000)/mL drained resin |
| BioProcess resin | Yes |
| Certificate of Analysis | Yes |
| Chemical stability | Stable in commonly used aqueous buffers, 1.0 M acetic acid, 1.0 M NaOH, 8 M Urea, 8 M guanidine hydrochloride, ethanol, methanol |
| Exclusion limit (M r ) (globular proteins) | 1000000 daltons |
| Flow velocity | < 50 cm/h |
| Ion Exchanger Type | Weak anion |
| Ionic Capacity | 0.10–0.14 mmol Cl - / mL resin |
| Particle size, d 50V | ~100 μm |
| Recommended Cleaning-in-Place Reagent | 2 M NaCl solution |
| Storage | 4 to 30°C, 20% Ethanol |
| pH ligand fully charged | < 9 |
| pH stability, CIP | 2–12 |
| pH stability, operational | 2–12 |
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