Cytiva, 17061201, Sephacryl S-100 HR, 750 mL

Name: Sephacryl S-100 HR, 750 mL
Brand: Cytiva
SKU: 17061201

Available in five porosity ranges, suitable for fast, high recovery separations at laboratory and industrial scale

Catalog Number: 17061201

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Category

Process-Scale Chromatography Resins

Pack Size750 mL

TagsChromatography resins Size exclusion resins Chromatography products resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Sephacryl™ High Resolution size exclusion chromatography resins allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by size exclusion chromatography at laboratory and industrial scale.
Purification over a wide molecular weight range
High reproducibility due to high stability
High recoveries
Well-suited to industrial-scale use
The five chromatography resins available have different porosity ranges. For peptides and small proteins, Sephacryl S-100 HR is the best choice. When fractionating proteins in the molecular weight ranges of 5 × 103 to 2.5 × 105 and 1 × 104 to 1.5 × 106, use Sephacryl S-200 HR and Sephacryl S-300 HR, respectively. Note that these ranges include monoclonal antibodies and serum proteins.
Sephacryl S-400 HR and Sephacryl S-500 HR are recommended for separating polysaccharides and other macromolecules with extended structures, and even small particles such as plasmids.
Differentiating specs (see data file for full specifications):
All five Sephacryl HR resins are available in prepacked HiPrep™ Sephacryl HR SEC columns. Each chromatography resin is available in two different prepacked column sizes; 16/60 (120 mL) and 26/60 (320 mL). HiPrep Sephacryl HR SEC columns provide the excellent purification properties of Sephacryl HR resins in a convenient, ready-to-use format. Find the prepacked columns here .

Specifications

General

BioProcess resin	Yes
Certificate of Analysis	Yes
Chemical stability	Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl
Fractionation range (M r ) globular proteins	1 × 10³–1 × 10⁵
Matrix	Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.
Particle size, d 50V	~50 µm
Pressure/flow characteristics	≥125 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20°C using buffers with a viscosity similar to water).
Storage	4 to 30°C, 20% Ethanol
pH stability, CIP	2–13
pH stability, operational	3–11

Ordering

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