Cytiva, 17070901, DEAE Sepharose Fast Flow, 500 mL

Name: DEAE Sepharose Fast Flow, 500 mL
Brand: Cytiva
SKU: 17070901

Catalog Number: 17070901

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Category

Process-Scale Chromatography Resins

Pack Size500 mL

TagsChromatography resins Ion exchange resins Chromatography products resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

DEAE Sepharose Fast Flow is a weak anion exchange chromatography resin (medium) for fast protein purification.
Well-proven weak anion exchanger developed for industrial downstream processes.
Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals.
High chemical stability allows for well proven CIP and sanitization protocols.
Hydrophilic base matrix ensures low levels of non-specific binding, and low levels of host cell-derived impurities in the elution pool.
Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Streamlined scale-up
This resin is part of the Sepharose Fast Flow ion exchange platform, which is well established in industrial downstream processing. Composed of crosslinked 6% agarose beads with diethylaminoethyl (DEAE) weak anion exchange groups, the resin has high chemical stability, allowing well-proven cleaning-in-place (CIP) and sanitization protocols.
The highly crosslinked agarose resin enables high flow rates and high productivity. Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, for example PreDictor 96-well plates, and PreDictor RoboColumns™, and HiTrap columns.
Successful use – research scale
DEAE Sepharose Fast Flow resin is used in a variety of research applications, including:
Separation of PI 3-Kinases from guinea pig neutrophils
Purification of myosin phosphatase from chicken smooth muscle
Compatible empty columns – research scale
Cytiva's solutions include a variety of empty columns for research scale. This DEAE resin is compatible with two of these lines, Tricorn and XK columns.
Compatible empty columns – bioprocess scale
For process-scale applications for this DEAE resin, Cytiva recommends AxiChrom columns. DEAE Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.
Prepacked columns – research scale
This weak anion exchange resin is available in the following formats:
HiScreen DEAE FF for scale up and process development HiPrep DEAE FF columns for convenient scale-up
HiScreen DEAE FF for scale up and process development
HiPrep DEAE FF columns for convenient scale-up
Prepacked columns – bioprocess scale
Prepacked ReadyToProcess columns for bioprocess applications are available on request.

Specifications

General

BioProcess resin	Yes
Certificate of Analysis	Yes
Chemical stability	Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Exclusion limit (M r ) (globular proteins)	~4 × 10⁶
Ion Exchanger Type	Weak anion exchanger
Ionic Capacity	0.12–0.15 mmol (Cl - )/mL resin
Ligand	Diethylaminoethyl
Matrix	cross-linked agarose, 6%, spherical
Particle size, d 50V	~90 µm
Pressure/Flow Specification	300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
Storage	4 to 30°C, 20% Ethanol
pH ligand fully charged	Below 9
pH stability, CIP	2–14
pH stability, operational	2–12

FAQs

Question: What type of ion exchanger is DEAE Sepharose Fast Flow?

Answer:A weak anion exchanger with diethylaminoethyl groups for binding negatively charged biomolecules.

Question: What is the supplied resin volume?

Answer:The package contains 500 mL of DEAE Sepharose Fast Flow chromatography resin.

Question: How are bound proteins typically eluted?

Answer:By increasing ionic strength or adjusting buffer conditions to reduce electrostatic binding.

Application Note

DEAE Sepharose™ Fast Flow is a weak anion-exchange chromatography resin for separating proteins and other charged biomolecules. Its diethylaminoethyl functional groups provide positively charged sites that bind negatively charged molecules under suitable pH and ionic strength conditions. The Sepharose Fast Flow agarose matrix supports preparative purification, allowing loading, washing and elution in scalable packed columns. Method development begins by selecting a buffer pH at which the target molecule and likely impurities have different charge behavior. After equilibration, the sample is applied at an appropriate conductivity; bound molecules can be recovered by increasing salt concentration or changing the buffer conditions. Monitor breakthrough and product recovery to determine loading capacity in the actual feed stream. Column cleaning and storage procedures must follow the resin's chemical compatibility limits. DEAE Sepharose Fast Flow is a weak anion exchanger and should not be confused with Q Sepharose Fast Flow, which uses a strong quaternary ammonium functional group.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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