Cytiva, 17081005, Phenyl Sepharose CL-4B, 10 L
Catalog Number: 17081005
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Pack Size10 L
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| Chromatography technique | Hydrophobic Interaction Chromatography |
|---|---|
| Autoclavable | 121°C for 30 min |
| Avoid Using | Oxidizing materials |
| Binding Capacity/ml Chromatography Medium | HSA (Mr 68 000) 15-20 mg/mL drained resin, ß-lactoglobulin (Mr 18 000) 3-5 mg/mL drained resin |
| Certificate of Analysis | Yes |
| Chemical stability | Stable in commonly used aqueous buffers and additives such as 8 M urea and 6 M guanidine hydrochloride. |
| Flow velocity | < 150 cm/h |
| Ligand | Phenyl |
| Ligand density | 40 μlmole/mL drained medium |
| Matrix | Cross-linked agarose, 4%, spherical |
| Particle Size | 45 μm-165 μm |
| Particle size, d 50V | ~90 μm |
| Sanitization Conditions | 0.5–1 M NaOH for at least 5 hours |
| Storage | 4 to 30°C, 20% Ethanol or 0.1 M NaOH |
| pH stability, CIP | 4–12 |
| pH stability, operational | 3–12 |
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