Cytiva, 17090202, ECH-Lysine Sepharose 4 Fast Flow resin, 500 mL
Catalog Number: 17090202
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Pack Size500 mL
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| Chromatography technique | Affinity coupling |
|---|---|
| Binding Capacity/ml Chromatography Medium | > 1.5 mg Plasminogen/mL drained resin |
| BioProcess resin | No |
| Certificate of Analysis | Yes |
| Chemical stability | Stable to commonly used aqueous buffers: 1 M NaOH, 6 M guanidine hydrochloride, |
| Coupling Chemistry | NHS |
| Ligand | L-lysine |
| Ligand Coupling Method | Amide linkage |
| Ligand density | ~ 16 µmol lysine/ml drained medium |
| Matrix | 4% cross-linked agarose |
| Order Information | This product is produced based on a customer order. Delivery time may be longer than for standard products. |
| Particle Size | 45 µm-165 µm |
| Particle size, d 50V | ~90 µm |
| Pressure/Flow Specification | Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
| Storage | 4 to 30°C, in 20% Ethanol and 0.05 M Sodium Acetate |
| pH stability, CIP | 2–13 |
| pH stability, operational | 3–12 |
Answer:Plasminogen and plasminogen activators.
Answer:Covalently coupled L-lysine on highly crosslinked 4% agarose.
Answer:500 mL of pre-swollen chromatography resin.
Cytiva ECH-Lysine Sepharose 4 Fast Flow is a group-specific affinity chromatography medium for isolation of plasminogen and plasminogen activators. L-lysine is covalently attached through a hydrophilic spacer to a highly crosslinked 4% agarose matrix, enabling selective affinity binding during protein purification. The resin has a mean particle size of 90 µm, a ligand density of 13–18 µmol lysine per mL of drained medium, and a working pH range of 3–12. The 500 mL pack contains pre-swollen medium in a buffered 20% ethanol solution. This material is part of Cytiva’s Custom Designed Media range rather than a routine prepacked laboratory column.
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