Cytiva, 17090602, NHS-Activated Sepharose 4 Fast Flow, 500 mL
High-performance NHS-activated resin for efficient covalent coupling of proteins, peptides, or other ligands.
Catalog Number: 17090602
Send an RFQ.
Pack Size500 mL
Loading…
| Chromatography technique | Affinity coupling |
|---|---|
| BioProcess resin | Yes |
| Certificate of Analysis | Yes |
| Chemical stability | Stable in commonly used aqueous buffers provided that the ligand withstand the pH or the chemical environment |
| Group To be Coupled | Primary amines |
| Ligand | N-hydroxysuccinimide (NHS) activated |
| Ligand density | ~16-23 µmol NHS/ml drained medium |
| Matrix | 4% cross-linked agarose |
| Particle Size | 45 μm-165 μm |
| Particle size, d 50V | ~90 µm |
| Pressure/Flow Specification | Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
| Storage | 2 to 8°C, 100% Isopropanol |
| pH stability, CIP | 2 (ligand dependent)–13 (ligand dependent) |
| pH stability, operational | 2–9 |
Answer:It is a 500 mL pack of NHS-activated Sepharose 4 Fast Flow supplied as a suspension in 100% isopropanol.
Answer:No. The NHS-activated support is intended for covalent immobilization of a user-selected primary-amine-containing ligand.
Answer:No. It is bulk activated coupling medium that requires ligand coupling, blocking, washing and suitable column packing before chromatography.
Cytiva NHS-activated Sepharose 4 Fast Flow is a preactivated agarose chromatography medium for covalent attachment of proteins, antibodies and other ligands containing accessible primary amino groups. N-hydroxysuccinimide ester groups on the matrix react with suitable ligands to create customized affinity purification media when the coupling and blocking steps are performed correctly. This package contains 500 mL of the activated medium supplied as a suspension in 100% isopropanol; it is not a prepacked column or an affinity resin with a predetermined binding ligand. Appropriate ligand concentration, coupling buffer, hydrolysis control and wash conditions influence coupling yield and subsequent capture performance. The resin must be prepared and packed into an appropriate chromatography device. Handle the isopropanol-containing suspension as a flammable solvent preparation and follow the specific coupling protocol.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
