Cytiva, 17097303, Phenyl Sepharose 6 Fast Flow (High Sub), 1 L
Catalog Number: 17097303
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Category
Pack Size1 L
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| BioProcess resin | Yes |
|---|---|
| Certificate of Analysis | Yes |
| Chemical stability | Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea |
| Ligand | Phenyl |
| Ligand Concentration | ~45 µmol phenyl/mL resin |
| Matrix | cross-linked agarose, 6%, spherical |
| Particle Size | 45 µm-165 µm |
| Particle size, d 50V | ~90 µm |
| Pressure/flow characteristics | 250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20⁰C using buffers with the same viscosity as water). |
| Storage | 4 to 30°C, 20% Ethanol |
| pH stability, CIP | 2–14 |
| pH stability, operational | 3–13 |
Answer:The high-substitution resin has a higher phenyl ligand density than the low-substitution version, which changes protein retention and selectivity during hydrophobic interaction chromatography.
Answer:A reduction in binding-promoting salt concentration commonly weakens hydrophobic interactions. Develop the salt gradient and elution conditions for the specific protein mixture.
Answer:The resin is supplied as a 1 L pack of Phenyl Sepharose 6 Fast Flow (High Sub) chromatography medium.
Phenyl Sepharose 6 Fast Flow (High Sub) is a hydrophobic interaction chromatography resin for protein fractionation and purification. Phenyl ligands are coupled to a highly cross-linked agarose matrix, and the high-substitution variant provides greater ligand density than the low-substitution version. Hydrophobic interactions strengthen as suitable salts increase the mobile-phase ionic strength; proteins can then be eluted by reducing the salt concentration or changing the elution conditions. Use screening experiments to identify salts, binding strength and recovery for the target protein, rather than transferring a fixed gradient from a different resin. The 1 L format is suited to preparative method development and scale-up in packed chromatography columns. Column packing, sample pretreatment and cleaning conditions should follow the resin's defined operating limits to maintain resolution and reproducibility over repeated cycles.
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