Cytiva, 17127903, rProtein A Sepharose Fast Flow resin, 200 mL

Name: rProtein A Sepharose Fast Flow resin, 200 mL
Brand: Cytiva
SKU: 17127903

Catalog Number: 17127903

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Category

Mixed-Mode Resins

Pack Size200 mL

TagsChromatography resins Chromatography products Affinity antibody resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

rProtein A Sepharose 4 Fast Flow is an affinity resin for antibody purification at laboratory and process scale.
For monoclonal and polyclonal antibody purification at both laboratory and process scale.
Well established among Protein A resins as excellent for monoclonal antibody (mAb) processes.
Free from animal-derived components.
Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Monoclonal and polyclonal antibody purification
rProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity.
Protein A resin characteristics
The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.
Streamlined scale up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.
Successful use in published research
Cytiva's protein A resins are used in a variety of applications, including:
Molecular characterization of an arthritogenic collagen peptide interacting with I-Ar
Compatible empty columns – research scale
Cytiva's solutions include a variety of empty columns for packing. rProtein A Sepharose resin is compatible for use with HiScale, Tricorn, and XK columns.
Compatible empty columns – bioprocess scale
For process-scale applications, Cytiva recommends AxiChrom columns. rProtein A Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.
Convenient prepacked columns – research scale
rProtein A Sepharose Fast Flow is also available in convenient prepacked formats:
rProtein A GraviTrap, for use with gravity flow
HiTrap rProtein A FF, for use with a syringe, pump, or chromatography system
Convenient prepacked columns – bioprocess scale
Prepacked ReadyToProcess columns are available on request, eliminating the need for column packing and cleaning validation.

Specifications

General

Chromatography technique	Antibody affinity chromatography
BioProcess resin	Yes
Certificate of Analysis	Yes
Chemical stability	Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol
Dynamic binding capacity, Q B10	~35 mg hIgG/mL resin
Ligand	Recombinant protein A (E.coli)
Ligand Coupling Method	Epoxy activation
Ligand density	6 mg rProtein A/ml medium
Matrix	Cross-linked agarose, 4%, spherical
Particle Size	60 µm-165 µm
Particle size, d 50V	~90 µm
Pressure/flow characteristics	150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20°C using buffers with the same viscosity as water)
Storage	2 to 8°C, 20% Ethanol
Total binding capacity	~50 mg hIgG/mL resin
pH stability, CIP	3–12
pH stability, operational	3–10

Ordering

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