Cytiva, 17513101, GSTrap FF column, 5 mL (pack of 1)

Name: GSTrap FF column, 5 mL (pack of 1)
Brand: Cytiva
SKU: 17513101

One-step GST-tagged protein purification with high yield

Catalog Number: 17513101

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Category

Protein Purification Columns

Pack Sizepack of 1

TagsPrepacked columns Chromatography products Affinity tagged protein

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The resin, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.

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