Cytiva, 17542154, HiTrap™ Protein Select™ column 5 × 5 mL

Cytiva™ Protein Select™ resin is an affinity chromatography resin for purifying any recombinant protein that does not have an affinity binding partner.
Simple affinity purification protocol for recombinant proteins.
One-step purification and tag cleavage without the need for proteases or other elution reagents.
Delivers a highly pure, native protein with no remaining tag amino acids after tag cleavage.
Can be processed under mild conditions, which means no exposure to conditions that cause protein aggregation or loss of biological activity.
Flexible, enabling the use in multiple expression systems.
About Cytiva™ Protein Select™ resin
Cytiva™ Protein Select™ resin is an affinity chromatography resin for purifying recombinant proteins using the self-cleaving Cytiva™ Protein Select™ tag. This technology simplifies tagged protein purification when used in research and standardizes purification during process development of any protein that does not have an affinity binding partner.
Request the sequence of Cytiva™ Protein Select™ tag now
A simple purification protocol
Purifying a protein tagged with Cytiva™ Protein Select™ tag is simple. One chromatography step does the affinity purification, a traceless tag cleavage, and the tag removal. The pure protein obtained is in its native state with no traces of the tag amino acids. The protocol does not require the use of a protease, and the same buffer can be used for equilibration, binding, wash, and elution.
Formats for research through to commercial manufacturing
Cytiva™ Protein Select™ resin is available in 25 mL, 100 mL, 500 mL, 1 L, and 5 L packs of bulk resins and in HiTrap™ 1 mL and 5 mL columns, which are well suited for research, process development and scaling up to commercial scales. Packing methods for Tricorn™, HiScale™, or AxiChrom™ chromatography columns are available.
The resin is shipped in 20% ethanol. We offer other custom prepacked column formats on request, including custom packed ReadyToProcess™ columns. Contact your Cytiva sales representative for more information.
Regulatory support files (RSF) will be available shortly at cytiva.com/RSF . For a Cytiva™ Protein Select™ ligand restricted license, contact your Cytiva sales representative for assistance and updates on availability.