Cytiva, 17546810, Capto SP ImpRes, 25 mL

Name: Capto SP ImpRes, 25 mL
Brand: Cytiva
SKU: 17546810

A strong cation exchange resin for reliable intermediate purification and polishing steps in a commercial setting.

Catalog Number: 17546810

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Category

Ion Exchange Resins

Pack Size25 mL

TagsChromatography resins Ion exchange resins Chromatography products resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

With a high binding capacity and small bead size, Capto™ SP ImpRes strong cation exchanger is a good choice for reliable and robust polishing steps in industrial purification.
High-resolution intermediate purification and polishing resin based on the well-established Capto™ platform with traditional ligands. Smaller particle size compared with Capto™ S.
Flexible process design due to large operational window of flow rates and bed heights.
High throughput purifications easy to optimize and scale-up.
Higher manufacturing productivity enables improved process economy.
Resin fulfills industrial demands for security of supply, robust performance, and regulatory support
Designed for large feed volumes
Capto™ SP ImpRes is a strong cation exchanger for the high-throughput intermediate purification and polishing steps of a wide range of biomolecules. This chromatography resin is part of an expanded high-resolution platform based on the high-flow agarose Capto™ product line.
By combining the high-flow characteristics of Capto™ resin with a small particle size, Capto™ SP ImpRes delivers excellent pressure-flow properties with impressive resolution. The ability to run at higher flow rates and higher bed heights also increases flexibility in process design.
Suits large-scale purification processes
Capto™ SP ImpRes is a BioProcess™ chromatography resin developed for large-scale purification of biopharmaceutical proteins. Based on the well-established Capto™ base matrix and with traditional ionic group ligands, it provides many opportunities for improved productivity and straightforward process development.
Prepacked columns and other formats
Capto™ SP ImpRes resin is available in the following formats:
Process development scale: prepacked
PreDictor™ filter plates , 96-well plates for high-throughput screening or process development.
PreDictor™ RoboColumn™ units, miniaturized columns for high-throughput process development.
HiScreen™ columns , optimized for method and process development.
Validation columns , scale-down columns for robust and reproducible process validation and process development (Tricorn™ format)
f (x) columns , precalibrated chromatography column for fast and accurate mechanistic modeling during downstream process development.
Lab scale: prepacked for manual purification
HiTrap™ columns , for easy use with a syringe, peristaltic pump, or chromatography system.
Lab scale: prepacked for automated purification and analysis
HiTrap™ columns , for easy use with a syringe, peristaltic pump, or chromatography system.
Process characterization: resin kits
Process Characterization Kit , designed for studying the potential impact that resin ligand density might have on the process outcome.
Process-scale purification: prepacked
ReadyToProcess™ columns , 1 L and larger columns for clinical manufacturing that minimize the need for cleaning and cleaning validation.

Specifications

General

Chromatography technique	Ion Exchange Chromatography
Avoid Using	Oxidizing agents, cationic detergents
Binding Capacity/ml Chromatography Medium	> 70 mg lysozyme, > 95 mg BSA
BioProcess resin	Yes
Certificate of Analysis	Yes
Chemical stability	Commonly used aqueous buffers, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol and 70 % ethanol
Ion Exchanger Type	Strong cation exchanger
Ionic Capacity	0.13-0.16 mmol H + /ml medium
Ligand	Sulfopropyl group
Matrix	High-flow agarose
Particle Size	36-44 μm (d 50V )
Pressure/Flow Specification	300 kPa at min. 220 cm/h, 1 m diameter column, 20 cm bed height
Storage	4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate
pH stability, CIP	3–14
pH stability, operational	4–12

FAQs

Question: What is the pack volume?

Answer:The package supplies 25 mL of bulk Capto SP ImpRes chromatography resin.

Question: What is the intended separation mode?

Answer:Strong cation exchange separates proteins through differences in electrostatic interaction with sulfopropyl groups.

Question: Is this resin useful for polishing?

Answer:Yes. Its small agarose beads support high-resolution intermediate purification and polishing separations.

Application Note

Capto SP ImpRes strong cation exchange resin provides a small-volume medium for developing protein polishing and high-resolution charge-based separations. The 25 mL pack is useful for packing short development columns, testing salt gradients and evaluating chromatographic selectivity before using larger resin quantities. Sulfopropyl groups on an agarose matrix interact with positively charged proteins, while approximately 40 µm beads provide resolution suited to separation of closely related molecular species. The chemistry is strong cation exchange, not the Protein A affinity chemistry used for primary antibody capture. Adjust pH to establish the target protein's charge state, then control conductivity during equilibration, loading and elution. Retention and peak shape depend on feed composition, gradient slope, residence time and column packing. This resin is particularly suitable for comparing impurities and product variants that differ in charge behavior. The supplied medium is unpacked resin and requires an appropriate chromatography column and validated buffer conditions.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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