Description
Capto™ L resin is designed for antibody fragment purification, when the fragment contains the kappa light chain. Cytiva also offers the new MabSelect™ VL resin , which offers higher binding capacity and more robust alkaline stability than Capto™ L resins.
Suitable for the capture of a wide range of antibody fragments such as Fabs, dAbs, and single-chain fragment variable (scFv) .
High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.
Enables a platform approach to antibody fragment purification.
Excellent productivity, process economy, and process design flexibility due to rigid matrix.
Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Capto™ L resin is available in the following prepacked formats: HiTrap™ Protein L prepacked columns, HiScreen™ Capto™ L prepacked columns, and PreDictor™ Capto™ L 96-well plates.
Affinity resin for antibody fragment purification
Capto™ L combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.
The high binding capacity and affinity to a broad range of antibody fragments makes this resin an excellent capture platform for antibody fragments containing kappa light chains. Common uses include domain antibody (dAb), scFv, and Fab purification.
Using this protein L resin
Capto™ L resin requires minimal optimization and produces high purity in one separation step, resulting in significant savings during process development. This resin is also advantageous in manufacturing, because high selectivity of affinity capture reduces the number of subsequent polishing steps.
Streamlined scale up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, such as PreDictor™ plates, PreDictor™ RoboColumn™, and HiScreen™ prepacked columns.
Successful use in published research
Capto™ L is used in applications requiring the purification of antibody fragments through protein L affinity chromatography.
Compatible empty columns – research scale
Capto™ L resin is compatible with empty PD gravity flow, Tricorn™, XK, and HiScale™ columns.
Compatible empty columns – bioprocess scale
For process-scale applications, Cytiva recommends AxiChrom™ columns.
Convenient prepacked columns – research scale
Capto™ L resin is available in the following formats:
HiTrap™ Protein L prepacked columns : convenient for easy use with a syringe, peristaltic pump, or chromatography system
HiScreen™ Capto™ L prepacked columns , for method and process development
PreDictor™ Capto™ L 96-well plates , for high-throughput screening or process development
Convenient prepacked columns – bioprocess scale
Prepacked ReadyToProcess™ columns are available, eliminating the need for column packing and cleaning validation.
