Cytiva, 27084601, Thrombin
Catalog Number: 27084601
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| Application | Used for site-specific separation of the GST tag from proteins expressed using pGEX-T vectors |
|---|---|
| Cleavage Buffer | PBS: 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.3 |
| Elution Buffer | 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0 |
| Inhibitor | Pefabloc TH benzamidine, AEBSF, APMSF, antithrombin III, Antipain, α1-antitrypsin, aprotinin, chymostatin, hirudin, leupeptin, PMSF |
| Molecular Weight (Mr) | 37000 |
| Supplied As | Lyophilized powder |
| Unit Definition | One unit of enzyme cleaves >= 90% of 100 μg of a test GST fusion protein when incubated in 1 × PBS at 22°C for 16 h. |
| Vector | pGEX-4T-1, pGEX-4T-2, pGEX-4T-3, pGEX-2TK |
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