Cytiva, 28903529, TempliPhi sequence resolver kit,20 reactions

Trends in rolling circle amplification: Capabilities for manipulation, error free production and assembly of nucleic acids are advancing. Find out how TempliPhi amplification kits use isothermal rolling circle amplification (RCA), enabled by phi29 DNA polymerase, to support these advancements . Description The kit can be used with both small (e.g., plasmids and M13) and large (fosmids and bacterial artificial chromosomes [BACs]) circular templates. The starting template for the reaction can be purified DNA, glycerol stock, liquid culture, or colonies. The reaction takes less than 20 min to prepare and after an overnight incubation at 10°C, it is ready to be used directly in sequencing applications with the sequencing chemistry of choice. A ten microliter (10 μl) reaction typically yields 1 μg of DNA in a standard overnight reaction.