Answer:It connects a floor-mounted AxiChrom 50–70 column with an ÄKTA pilot chromatography system.
Answer:The kit is specified with a 1.7 mm inner diameter. A kit for larger-bore connections is a different configuration.
Answer:Match the column ports and pilot-system valves to the connection guide, then inspect the assembled path for leakage, incorrect routing and unnecessary dead volume.
The AxiChrom tubing kit connects floor-mounted AxiChrom 50–70 chromatography columns to the ÄKTA pilot system in preparative protein purification workflows. The fluid path has a 1.7 mm inner diameter, allowing the specified configuration to be integrated into the column and system connections without substituting larger-bore tubing intended for other AxiChrom models. Route the tubing using the column connection guide and verify the direction of each port before introducing sample. After assembly, flush the lines with suitable liquid, check fittings for leakage, and monitor pressure during the initial run. A pre-run assessment should cover dead volume, sample carryover and the compatibility of wetted materials with process buffers. Different AxiChrom column sizes and installation positions require different connection sets, so selecting tubing by column diameter alone is insufficient.
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