Cytiva, 29-0588-05, Antibody package, Protein A

Comprehensive human antibody purification HiTrap Protein A HP is prepacked with Protein A Sepharose High Performance. This resin is used for routine immunoaffinity purifications of most IgG from ascites, serum, and cell culture supernatant. These HiTrap columns deliver high-performance, high-resolution purifications with a binding capacity of 20 mg IgG/mL resin. For quick pH neutralization of the eluted antibodies, the package also includes a HiTrap Desalting column. These columns are prepacked with Sephadex G-25 Superfine for high sample recovery and minimal dilution. Other column kits for protein capture and buffer exchange Life Sciences solutions from Cytiva include other column packages for use with a chromatography system: Tagged Package for purification of GST-tagged proteins; Untagged Package for purification by cation or anion exchange followed by desalting; Antibody Package, Protein G for general-purpose antibody purification.