Cytiva, 29048641, MBPTrap HP column, 1 x 1 ml
Convenient, high binding capacity columns for MBP-tagged protein purification
Catalog Number: 29048641
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Pack Size1 x 1 mL
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Answer:The dextrin affinity medium captures proteins bearing a maltose-binding protein (MBP) fusion tag.
Answer:One prepacked column containing 1 mL of Dextrin Sepharose High Performance medium is supplied.
Answer:Competitive elution with maltose can release bound MBP-fusion proteins; optimize the buffer composition and confirm target recovery for the specific construct.
MBPTrap HP is a prepacked affinity chromatography column for capturing proteins fused to a maltose-binding protein (MBP) tag. Its 1 mL bed of Dextrin Sepharose High Performance provides a dextrin-based binding surface for MBP-tagged recombinant proteins. This single-column format is useful for small-scale purification, expression screening and optimization of sample loading, washing and elution. Following sample clarification, adjust the binding buffer to preserve fusion-protein solubility and monitor target recovery across the flow-through, wash and elution fractions. Maltose-based competitive elution can release bound fusion proteins under appropriately selected conditions. Evaluate the purity and identity of collected fractions using suitable downstream analytical methods.
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