Cytiva, 29400458, HiTrap Capto S, 1 × 1 mL
Catalog Number: 29400458
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Category
Pack Size1 mL
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Answer:Sulfoethyl groups provide strong cation-exchange functionality for binding positively charged proteins under suitable buffer conditions.
Answer:One prepacked HiTrap column with a 1 mL resin bed is supplied.
Answer:Yes. The 1 mL format supports comparison of loading, washing and salt-elution conditions on appropriately configured liquid chromatography equipment.
HiTrap Capto S is a prepacked strong cation-exchange chromatography column for the capture, intermediate purification and method development of proteins carrying a net positive charge under the selected buffer conditions. The 1 mL column contains Capto S resin with sulfoethyl functional groups on a robust agarose-based matrix. This compact format supports screening of binding pH, salt concentration and elution conditions before transferring an optimized process to larger columns. Clarify the sample, equilibrate the column in a suitable low-conductivity buffer and collect breakthrough and elution fractions for analysis. Protein selectivity depends on the relationship between working pH, sample ionic strength and the target protein’s charge properties.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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