Cytiva, 29497629, Antibody column package, modern Protein A (2-step purification protocol)

Protein A affinity chromatography captures your antibody The kit includes a HiTrap column prepacked with MabSelect PrismA resin containing a protein A-derived ligand produced in E. coli . The ligand has been specially engineered to create an affinity resin with enhanced alkali and protease stability. The specificity of binding to the Fc region of IgG is similar to conventional Protein A and provides you with excellent purification in one step. High final antibody purity through size exclusion chromatography HiPrep 16/60 Sephacryl S-300 HR size exclusion chromatography column is prepacked with Sephacryl S-300 HR chromatography resin, which has a broad fractionation range of M _r 10 000 to 1 500 000 for globular proteins. The resin is well-suited for antibody purification and the high sample capacity of the HiPrep column lets you load samples of up to 5 mL. Find out more how to set up a purification protocol for antibodies.