Description
Request a sample Today tens of thousands of individual cells from a single tissue sample or patient can be analyzed, giving researchers the opportunity to understand individual cell populations and their behavior in diseased tissue. One of the first and most critical steps in single cell analysis is the dissociation of tissues to yield fully dissociated but intact and viable cells. Single-cell preparations of the tissues are quite cumbersome because the lung, liver, brain and kidney are complex organs, both functionally and anatomically. Effective enzymatic and mechanical dissociation of lung, liver, brain and kidney tissue is necessary to complete single-cell analysis so that targeted treatments can be developed. Cold-temperature tissue dissociation is becoming more popular because it is thought to better preserve the natural transcriptome. Read more about the single-cell RNA sequencing and cold dissociation *For research use only. **Performance data is not yet available for Enzyme mix D (for kidney) using manual methods or at temperatures of 37⁰C. Enzyme mix B (for liver) is recommended for use at 37⁰C as liver is very sensitive to cold. Performance data is not available for Enzyme mix A (for lung) using semi-automated methods at temperatures of 37⁰C. Performance data is not available for Enzyme mix C (for brain) using semi-automated methods at temperatures of 37⁰C. Data is available for all other enzymes in the range.
