Cytiva, BR100354, Glycine 1.5

The Cytiva Biacore glycine-HCl regeneration solution removes residual bound analyte from suitable surface plasmon resonance sensor surfaces between binding cycles. This ready-to-use 10 mM glycine-HCl reagent has a pH of 1.5 and is supplied in a 100 mL bottle. A low-pH regeneration pulse disrupts certain noncovalent molecular interactions, allowing the sensor surface to be returned toward a consistent baseline before the next analyte injection. Regeneration efficiency and immobilized ligand stability must be assessed together: conditions that remove analyte effectively can also affect sensitive ligands. Method setup should determine a contact time and flow strategy that provide reproducible regeneration without unnecessary exposure. Verify baseline recovery and ligand activity across repeated cycles, and compare alternative regeneration conditions when the bound complex is especially robust or fragile. This solution is part of a Biacore SPR method workflow, not a sample-running buffer or sensor-chip coating reagent. It should not be used as a generic protein purification eluent without an independently validated procedure.