Cytiva, BR100633, Amine Coupling Kit, type 2

The Biacore Amine Coupling Kit Type 2 supplies chemical reagents for covalent immobilization of primary-amine-containing ligands on compatible surface plasmon resonance sensor chips. In a standard carboxyl-surface procedure, activated surface groups react with exposed amino groups of a protein, peptide or other ligand, creating a stable surface suitable for repeated analyte injections. Ethanolamine blocking terminates remaining reactive sites and helps reduce unwanted interactions. This type 2 kit supports approximately 60–80 immobilization procedures, making it suitable for laboratories preparing multiple ligand surfaces in kinetic, affinity or screening studies. Immobilization level depends on ligand concentration, buffer pH, contact time and chemical accessibility of primary amines; higher loading is not necessarily better for quantitative kinetics. Include a reference surface and evaluate nonspecific binding before running a sample series. Reagents do not replace the sensor chip, instrument running buffer or ligand material. Prepare and handle the kit components using the appropriate amine coupling method.