Cytiva, RPN8501, Nucleon BACC1

Name: Nucleon BACC1
Brand: Cytiva
SKU: RPN8501

Rapid extraction of high molecular weight genomic DNA from whole blood and cultured cells in around 30 min, with no organic solvents.

Catalog Number: RPN8501

Pricing

Send an RFQ.

View RFQ List

Category

Genomic DNA Isolation Kits

TagsMolecular and Immunodiagnostics Extraction kits

CollectionsCytiva Molecular & Immunodiagnostics Collection Cytiva Protein Analysis Equipment & Supplies Collection

Description

Nucleon BACC system, genomic DNA isolation kit There are many methods of purifying, extracting or isolating genomic DNA, and illustra Nucleon BACC Genomic DNA Extraction Kits are particularly well-adapted to the need for standardization when analyzing multiple routine samples, such as blood, cell culture, and tissue samples in core facilities. Nucleon BACC Genomic DNA Extraction kits use a proprietary resin which is added following cell lysis, deproteinization with sodium perchlorate, and a single chloroform extraction, for a phenol-free protocol. The combination of purity and high molecular weight makes the DNA from Nucleon BACC kits suitable in multiple downstream molecular biology applications, including whole genome amplification prior to whole genome sequencing, or NGS sequencing.

FAQs

Question: What samples can be processed using the Nucleon BACC1 genomic DNA kit?

Answer:The kit processes whole blood and cultured cells for high-molecular-weight genomic DNA isolation.

Question: How many DNA extractions does the Nucleon BACC1 kit support?

Answer:The kit is specified for 25 preparations.

Question: Does Nucleon BACC1 use a spin-column membrane?

Answer:No. It is a resin-assisted, non-column extraction system that removes proteins during phase separation.

Application Note

The illustra™ Nucleon™ BACC1 genomic DNA extraction kit isolates high-molecular-weight DNA from whole blood and cultured cells using a resin-assisted, non-column extraction workflow. This configuration provides 25 preparations and is intended for smaller inputs such as 50 µL to 1.0 mL of blood or approximately one to three million cultured cells. Following lysis and protein removal, Nucleon resin forms a separable phase that facilitates recovery of the DNA-containing aqueous fraction. The chemistry supports preparation of genomic DNA for downstream molecular analysis, including PCR and other assays requiring intact DNA. The kit includes extraction reagents A and B, 5 M sodium perchlorate and Nucleon resin. The phenol-free workflow uses a chloroform extraction and additional ethanol steps. Those solvents must be supplied separately for the complete procedure.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.