Answer:1 M in a 100 mL bottle.
Answer:HEPES helps stabilize medium pH despite changes in dissolved carbon dioxide.
Answer:pH 7.2–7.5, with refrigerated storage at 2–8 °C.
Cytiva HyClone liquid HEPES buffer is a concentrated pH-stabilizing supplement for cell culture media. The 1 M solution is supplied in a 100 mL bottle and supports buffering when dissolved carbon dioxide and cellular metabolism cause changes in the culture environment. The HEPES formulation is a convenient liquid source for supplementing compatible basal media during cell maintenance and experimental preparation. The sterile-filtered solution has a specified pH of 7.2–7.5 and is stored refrigerated at 2–8 °C. It is a buffering reagent rather than a complete nutrient medium.
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