Cytiva, SH30933.01, HyClone™ HyCell™ CHO powder medium without L-glutamine and with HT, 5 L

Cytiva HyClone HyCell CHO Powder Medium is a chemically defined, animal-derived component-free culture medium intended for Chinese hamster ovary cells in recombinant protein production. The 5 L powder format is reconstituted to its specified final medium volume using qualified water and a controlled preparation procedure. This formulation contains hypoxanthine and thymidine (HT) but does not contain L-glutamine. HT supplementation is relevant to compatible production cell lines and should be distinguished from formulations designed without HT for particular selection systems. The glutamine strategy must be matched to the cell line; a separate supplement may be needed at the validated concentration. The medium supports development of fed-batch and perfusion culture processes, but actual cell growth and production depend on feed strategy, clone and operating conditions. During preparation, ensure full dissolution, appropriate pH and osmolality, and a suitable filtration and storage workflow. The 5 L designation is a preparation volume rather than a ready-to-use liquid bottle.