Cytiva, SH30933.03, HyClone™ HyCell™ CHO powder medium without L-glutamine and with HT, 50 L

Cytiva HyClone HyCell CHO Powder Medium is a chemically defined and animal-derived component-free basal medium for CHO cell culture and recombinant protein production. This powder presentation yields 50 L of prepared medium, making it appropriate for larger media-preparation batches rather than a single ready-to-use bottle. The specified formula contains hypoxanthine and thymidine (HT) and omits L-glutamine. These nutritional features matter when transferring a process between cell clones or changing from a selection-phase medium to a production medium. Reconstitute the powder with qualified water using controlled mixing and established dissolution instructions; assess pH, osmolality and sterile-filtration compatibility before use. A separate glutamine supplement may be required depending on the cell line and the selected culture strategy. The medium can be considered for batch, fed-batch and perfusion workflows when supported by development data. Compared with the smaller 5 L presentation, this version changes the intended batch preparation volume, not the named nutrient formulation.