Answer:One dry powder pack prepares 5 L of Cell Boost 7a feed after hydration.
Answer:7a supplies nutrients including glucose and trace elements; 7b provides a concentrated alkaline amino acid feed.
Answer:No. Add the prepared feeds separately to the bioreactor to avoid precipitation.
HyClone™ Cell Boost™ 7a is a concentrated, chemically defined feeding supplement for Chinese hamster ovary (CHO) cultures producing recombinant proteins. This dry powder package prepares 5 L of liquid feed after hydration and is designed for fed-batch processes based on ActiPro™ or ActiSM™ media. Cell Boost 7a supplies glucose, amino acids, vitamins, salts, and trace elements, helping replenish nutrients consumed during high-density cell growth and antibody production. It is animal-derived component-free and contains no protein hydrolysates or added growth factors. Cell Boost 7a complements the separate Cell Boost 7b amino acid feed; both solutions enter the bioreactor independently to avoid precipitation. A 10:1 volume ratio of 7a to 7b provides a starting point for feed development, with dosing adapted to the CHO clone.
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