Answer:It is a dry powder supplement intended to yield 10 L of feed solution after proper reconstitution.
Answer:It is designed for fed-batch CHO cell culture, including workflows using HyClone ActiPro and ActiSM media.
Answer:No. Add the feeds separately into the culture; mixing the concentrated supplements together beforehand may produce precipitate.
Cytiva HyClone Cell Boost 7a is a chemically defined, animal-derived component-free nutrient feed supplement supplied as dry powder for preparation of 10 L of final solution. It supports fed-batch Chinese hamster ovary cell culture, particularly processes using HyClone ActiPro or ActiSM media for recombinant protein production. The formulation provides nutrients including amino acids, vitamins, inorganic salts, trace elements and glucose. The 10 L designation identifies the volume after reconstitution, not the weight of the powder or a prefilled liquid container. Cell Boost 7a is used alongside the complementary Cell Boost 7b supplement, but the concentrated solutions should be added separately rather than premixed because precipitation may occur. Prepare the feed with qualified water using the validated mixing, pH adjustment and filtration procedure. Establish an appropriate feed schedule by tracking viable cell density, culture metabolites and product quality. Protect prepared material under the specified storage conditions and perform process-specific suitability testing before manufacturing use.
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