Cytiva, SR30004.KT, HyCell-STEM-FF media kit; includes 100 mL 6x supplement and 500 mL DMEM/F12

This defined, serum-free formulation is used for expanding and maintaining stem cell colonies on feeder layer cultures with daily or every other day feeds, resulting in increased cell growth with fewer passages, all while maintaining pluripotency.Includes: HyCell-STEM kit includes 6x supplement and DMEM/F12 to create a complete media. Basic fibroblast growth factor must be added to the completed media at time of use. Storage Conditions Upon receipt, store HyCell-STEM 6X supplement frozen (-20°C) and DMEM/F12 at 2° to 8°C. Before use, thaw 6X supplement overnight at 2° to 8°C. Avoid repeated freeze/thaw of supplement. Required Accessories bFGF (10ng/ml) must be added to the media at time of use.