Description
DURAN® membrane venting screw caps can be used for safer autoclaving of media, or in cell incubator culture requiring high rates of gas exchange. The screw cap can be tightly closed as the membrane permits an automatic sterile gas exchange, allowing safe pressure equalisation during the autoclave cycle, and at the same time ensuring the sterility of the bottle contents. Large exposed sterile-filter surface area allows efficient gas exchange rates (a pressure differential of 0.1 Bar is required) for cell culture applications in bottles and shaker flasks. DURAN® Original GL Screw Capped Laboratory bottles are ideal containers for sterilising and storing sterile media or buffer solutions. However, it is strongly recommended that during the autoclaving cycle the caps are only loosely tightened. Heating times are reduced if the steam is easily able to penetrate the contents of the bottle. Loose caps also reduce the risk of failure due to pressure build up inside the bottle, which can reach 400 kPa (absolute). In addition, tightening the screw caps immediately after autoclaving, will result in a vacuum caused by the cooling of the steam. This can lead to bottle failure, and is an especially important consideration for larger bottles. To overcome these limitations, the sterile venting membrane screw caps were developed. Membrane caps with hydrophobic PTFE membranes, facilitate optimal gas exchange by allowing gases like oxygen and carbon dioxide to pass through while preventing microbial contamination.
