Description
TruSight Tumor 15
Focused next-generation sequencing (NGS) panel to assess 15 commonly mutated genes in solid tumors with one simple and rapid workflow.
TruSight Tumor 15 Data sheet PDF < 1 MB
Data sheet PDF < 1 MB
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Overview
TruSight Tumor 15 is an NGS assay that assesses 15 commonly mutated genes in solid tumors.
Accurate, economical, and rapid assessment of 15 genes in a single assay 1
Detection of somatic variants at 5% allele frequency using 20 ng DNA from FFPE tissue 1
Easy-to-interpret report requiring minimal bioinformatics resources
Seamless integration into existing lab practices
Relevant gene content
Content is carefully selected to include genes and gene regions cited by industry organizations and informed by independent consortia publications and late-stage pharmaceutical research. Content includes single nucleotide variants (SNVs) and indels with demonstrated involvement in solid tumors.
Comprehensive workflow
The TruSight Tumor 15 assesses 15 genes in a simple, rapid workflow that replaces single, iterative gene testing with polymerase chain reaction (PCR). Analyze data with on-instrument reporting software or the TruSight Tumor 15 BaseSpace Sequence Hub App .
Accelerated tumor profiling
Go from DNA to data in ~36 hours with only 3.5 hours of hands-on time. Maximize sample success rates across multiple tumor types to conserve limited samples and resources.
Applications
TruSight Tumor 15 is a comprehensive workflow solution that assesses somatic variants in 15 genes commonly associated with solid tumors in a single assay, enabling labs to use the power of NGS to focus on genes most likely to play a role in tumorigenesis and confidently analyze low-frequency variants from FFPE DNA.
Example workflow
TruSight Tumor 15
MiniSeq System
MiSeq System
Local Run Manager software (TruSight Tumor 15 module)
TruSight Tumor 15 BaseSpace Sequence Hub app
Project recommendations
Instrument: Recommended number of samples
MiniSeq System: Samples per run: mid output: 2-3, high output: 8 (based on 93.5% of the bases covered at 500x minimum)
Samples per run: mid output: 2-3, high output: 8 (based on 93.5% of the bases covered at 500x minimum)
2 × 150 bp (max recommended)
MiSeq System: 8 samples per run with v3 reagents (based on 93.5% of the bases covered at 500x minimum)
8 samples per run with v3 reagents (based on 93.5% of the bases covered at 500x minimum)
2 × 151 bp (max recommended)
Related applications and methods
Cancer sequencing methods
NGS-based cancer sequencing methods provide more information in less time compared to single-gene and array-based approaches.
Pathology and clinical cancer research
Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.
Amplicon sequencing
Ultra-deep sequencing of PCR amplicons enables analysis of specific genomic regions of interest. Learn more about amplicon sequencing and find comprehensive solutions.
TruSight Tumor 15 chemistry
The TruSight Tumor 15 assay uses a multiplex PCR approach, resulting in high accuracy and sensitivity.
TruSight Tumor 15 Gene List
AKT1: GNA11
BRAF: GNAQ
EGFR: KIT
ERBB2: KRAS
FOXL2: MET
TruSight Tumor 15 MiSeq Kit
OP-101-1001
Includes 1 library prep kit and 3 MiSeq reagents sufficient for 24 samples. View less
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TruSight Tumor 15 (Library Prep Only)
OP-101-1002
Includes 1 library prep kit sufficient for 24 samples. View less
TruSight Tumor 15 MiniSeq Kit
20005610
Includes 1 library prep kit and 3 MiniSeq reagents sufficient for 24 samples. View less
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Which Illumina sequencing systems are compatible with TruSight Tumor 15?
TruSight Tumor 15 is supported on the MiniSeq System and MiSeq System (v3 chemistry), as well as on the MiSeqDx Instrument (in Research Mode; v3 chemistry). Sequencing on other Illumina systems is possible but has not been tested.
Which thermal cyclers have been validated for use with TruSight Tumor 15?
The following thermal cyclers are recommended for use with the TruSight Tumor 15 kit. Follow the recommended ramp rate setting listed in the TruSight Tumor 15 Reference Guide.
Bio-Rad C1000
Bio-Rad S1000
Applied Biosystems GeneAmp PCR System 9700
Applied Biosystems Veriti Thermal Cycler
Eppendorf Mastercycler ep Gradient
Eppendorf Mastercycler ep Gradient-S
Bio-Rad C1000, Applied Biosystems GeneAmp PCR System 9700, and Eppendorf MasterCycler ep Gradient were qualified internally
What is the difference between TruSight Tumor 15 and TruSight Tumor 170?
TruSight Tumor 15 targets 15 commonly mutated genes in solid tumors using amplicon sequencing and targeted DNA sequencing on the MiniSeq, MiSeq, and MiSeq Dx (v3 chemistry) Systems. TruSight Tumor 170 targets 170 genes using target enrichment and targeted DNA and RNA sequencing on the NextSeq 500 and NextSeq 550 Systems.
Are sample BaseSpace Sequence Hub data sets available?
Yes, you may view a sample run and a sample project . A BaseSpace Sequence Hub login is required to access this data.
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1. Data calculations on file. Illumina, Inc. 2016.
