Illumina, 20015963, TruSeq DNA PCR-Free High Throughput Library Prep Kit (96 samples)

Overview
TruSeq DNA PCR-Free provides simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to human whole genomes. The workflows offer:
Shortened gel-free workflows that remove the need for PCR
Ability to sequence challenging regions
Excellent coverage quality for deep insight into the genome

Sequence challenging regions
TruSeq DNA PCR-Free offers superior coverage of areas that are traditionally difficult to sequence, such as GC-rich regions, promoters, and repetitive content.
The workflows are tunable to a variety of read lengths and are supported on all Illumina sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Gain deep insight into the genome
PCR-free means reduced library bias and gaps. The result is high data quality and optimal variant detection across the genome. Excellent genome coverage quality means your results have few gaps and good coverage of GC-rich regions.

Save time with a PCR-free protocol
Removing PCR saves time and removes genomic coverage bias associated with PCR steps. Bead-based size selection shortens the workflow. In tandem with Illumina sequencing systems, TruSeq DNA PCR-Free provides a range of enhancements to a widely adopted library preparation workflow.

Access flexible throughput options
TruSeq DNA PCR-Free with Single Indexes supports 24-sample manual processing for low-throughput (LT) studies.
TruSeq DNA PCR-Free with 96 CD Indexes supports 96-sample processing for high-throughput (HT) studies, and can be automated on liquid-handling robots (or processed manually).
IDT for Illumina Unique Dual (UD) Indexes (24 or 96) offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell.

Fast, Automation-friendly Workflow
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners .

Example workflow
Whole-Genome Sequencing
NextSeq 550 System
NovaSeq 6000 System
BaseSpace BWA Aligner App
BaseSpace Isaac WGS App
BaseSpace SPAdes Genome Assembler App

Project recommendations
Instrument: Recommended number of samples
NextSeq 550 System: 1 sample per run (based on 30× coverage of a human genome)
1 sample per run (based on 30× coverage of a human genome)
350 bp insert: ≤ 2 × 100 bp 550 bp insert: ≤ 2 × 150 bp
NovaSeq 6000 System: Samples per run (dual flow cell): S1: 8, S2: 16, S4: 48 (based on 30× coverage of a human genome)
Samples per run (dual flow cell): S1: 8, S2: 16, S4: 48 (based on 30× coverage of a human genome)
350 bp insert: ≤ 2 × 150 bp 550 bp insert: ≤ 2 × 150 bp

Related applications and methods
Large Whole-Genome Sequencing
Large whole-genome sequencing informs disease research and population genomics studies and reveals disease-associated alleles.
Microbial Whole-Genome Sequencing
Use microbial whole-genome sequencing to map genomes of novel organisms, finish genomes of known organisms, or compare genomes across multiple samples.
Researchers from the Max Planck Institute and the University of Pennsylvania crowdfunded the genome sequencing of Lil Bub, using the NextSeq 500 System to uncover the genetic causes of the cat’s rare diseases.