Illumina, 20015964, TruSeq Nano DNA Low Throughput Library Prep Kit (24 samples)

Overview
TruSeq DNA Nano enables efficient interrogation of samples with limited available DNA. Based on mechanical fragmentation–ligation chemistry, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

Key features
Designed for low sample input
High coverage quality
Accelerated library prep

Manual preparation of high-quality libraries in less than a day
TruSeq DNA Nano has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.

Use with limited DNA samples
TruSeq DNA Nano offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

Reduce library bias and coverage gaps
TruSeq DNA Nano reduces the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of traditionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. Truseq DNA Nano products are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

Access flexible throughput options
TruSeq DNA Nano with Single Indexes supports 24-sample manual processing for low-throughput (LT) studies.
TruSeq DNA Nano with 96 CD Indexes supports 96-sample processing for high-throughput (HT) studies, and can be automated on liquid-handling robots (or processed manually).
IDT for Illumina Unique Dual (UD) Indexes (24 or 96) offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell.

Fast, Automation-friendly Workflow
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners .

Example workflow
Whole-genome sequencing
NextSeq 550 System
NovaSeq 6000 System
BaseSpace BWA Aligner App
BaseSpace Isaac WGS App
BaseSpace SPAdes Genome Assembler App

Project recommendations
Instrument: Recommended number of samples
NextSeq 550 System: 1 sample per run (based on 30× coverage of a human genome)
1 sample per run (based on 30× coverage of a human genome)
350 bp insert: ≤ 2 × 100 bp 550 bp insert: ≤ 2 × 150 bp

Related applications and methods
Cancer whole-genome sequencing
Cancer whole-genome sequencing provides researchers with a base-by-base view of the unique mutations and abnormalities present in cancer tissue.
Large whole-genome sequencing
Large whole-genome sequencing informs disease research and population genomics studies and reveals disease-associated alleles.
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