20019102, AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

Overview
The AmpliSeq for Illumina BRCA Panel is a targeted resequencing assay for research of somatic and germline variants across BRCA1 and BRCA2 genes.

Key features
Relevant gene content - Target all exonic regions and flanking intronic sequences of BRCA1 and BRCA2
Fast, streamlined workflow - Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples
Accurate data - Detect somatic mutations as low as 5% variant allele frequency using local or cloud based analysis
The BRCA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
BRCA1 and BRCA2 are human tumor suppressor genes that, when carrying specific mutations, have been implicated in an increased risk for breast and ovarian cancers. This ready-to-use panel saves researchers the time and effort of identifying targets, designing primers, and optimizing panels.

Example workflow
AmpliSeq for Illumina BRCA Panel
AmpliSeq for Illumina Library PLUS
AmpliSeq for Illumina CD Indexes
iSeq 100 System
MiniSeq System
MiSeq System
BaseSpace Sequence Hub DNA Amplicon App
Local Run Manager DNA Amplicon Module

Project recommendations
Instrument: Recommended number of samples
iSeq 100 System: 12 samples per run (assumes minimum coverage of 500×) 96 samples per run (assumes minimum coverage of 50x)
12 samples per run (assumes minimum coverage of 500×) 96 samples per run (assumes minimum coverage of 50x)
2 × 150 bp 2 × 150 bp
MiniSeq System: Samples per run: mid output: 24, high output: 80 (assumes minimum coverage of 500×)
Samples per run: mid output: 24, high output: 80 (assumes minimum coverage of 500×)
2 × 150 bp
MiSeq System: Samples per run at 500x minimum coverage (by reagent kit version): v2 nano: 3, v2 micro: 12, v2: 48, v3: 80 Samples per run at 50x minimum coverage (by reagent kit version): v2 nano: 32, v2 micro: 96
Samples per run at 500x minimum coverage (by reagent kit version): v2 nano: 3, v2 micro: 12, v2: 48, v3: 80 Samples per run at 50x minimum coverage (by reagent kit version): v2 nano: 32, v2 micro: 96

Related applications and methods
Targeted resequencing
With targeted resequencing, a subset of genes or a genomic region is isolated and sequenced, which can conserve lab resources.
Pathology and clinical cancer research
Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.